What is the semaglutide content on the agreed reporting basis?
Separate the labelled vial amount from the amount of powder present. If the supplier specifies a salt form or a particular assay basis, include that information with the request so the content result can be compared appropriately.
Information to include
State the labelled semaglutide amount and any excipients. For a solution, include the diluent, total volume and preparation date if known.
A complete unopened vial is strongly preferred when content is being measured
State the nominal fill in milligrams and whether it is peptide base or gross powder
Do not reconstitute before shipping unless the laboratory has approved it in writing
Method and reporting scope
Roughly 1.5 to 5 kDa. Identity rests on a whole-spectrum comparison against reference data rather than a single reading, and related substances sit closer to the parent peak.
Area purity by HPLC-DAD, identity against reference spectra, verifiable COA. Reference availability and sample suitability are confirmed before acceptance. Read the method and reporting information for the techniques in your quote.
Price, timing and submission
The starting panel is $299 CAD. Volume rates, optional fees and tax are shown separately. Standard peptide analysis typically takes 5-10 business days after receipt and acceptance; other scopes are confirmed in the quote.
Testing Semaglutide: what the analysis has to get right
Semaglutide testing comes down to three separate questions: is it semaglutide, how pure is it, and is the labelled amount actually in the vial.
Semaglutide is a 31-residue GLP-1 analogue with a fatty diacid side chain and a modified amino acid that protects it from enzymatic breakdown. Its formula is C187H291N45O59 and its average molecular mass is 4113.58 g/mol.
Like other lipidated peptides it is well retained on reversed-phase columns and prone to surface adsorption, and its related impurities can sit close to the main peak.
What makes Semaglutide hard to measure
Resolving related substances
Truncated, deamidated and oxidised forms can co-elute under a generic fast gradient. Area purity is only as good as the separation behind it.
Identity against reference spectra
Semaglutide is confirmed by comparison against reference spectra, using a selection of FTIR-ATR, Raman spectroscopy, capillary electrophoresis with UV detection and UV-Vis spectroscopy chosen to suit the molecule. The comparison is only as specific as the expected identity named on the request.
Salt form and basis
Content can be expressed as free peptide or on another basis. The certificate should state which, or two correct results will not compare.
Solutions
For a liquid sample, the diluent, volume and preparation date matter as much as the label, because they change what is measured.
Which test answers which question
Is it semaglutide?
Identity confirmed against reference spectra.
How clean is it?
HPLC-DAD area purity with the method conditions stated and the chromatogram attached.
Is the label right?
Measured content against the label, on request where a reference standard is available.
Contaminants
Endotoxin and heavy metals as separate, optional tests.
Before you ship
Send the sealed vial as received. If the sample is already a solution, include the diluent, total volume and date so the content result can be interpreted.
Reading a Semaglutide certificate
Confirm the identity section says what the confirmation was made against, and by which techniques.
Check the content basis is stated (free peptide or otherwise) on any content result.
Read the content figure, not only purity, to judge the label claim.
Canada Peptide Testing accepts semaglutide samples by mail from anywhere in Canada and reports identity and purity on one verifiable certificate, with content added on request.
What should a semaglutide certificate include?
A named identity confirmation and the techniques behind it, HPLC purity with method conditions and a chromatogram, any content result with its basis, the lot, dates, and a way to verify the report with the laboratory.
Can I test a semaglutide solution?
Yes, but include the diluent, total volume and preparation date. The result describes the solution received, not the original powder.
