Is this a tirzepatide-only sample or a combination?
For a tirzepatide-only vial, request the accepted purity, identity and content scope. A product combining tirzepatide with cagrilintide or another ingredient needs each expected component listed separately, including any stated ratio.
Also listed as: LY3298176. Confirm the intended form from the specification.
Information to include
Provide the amount of tirzepatide per vial, other labelled ingredients and any prior dilution. A combined blend mass should not be entered as tirzepatide mass.
A complete unopened vial is strongly preferred when content is being measured
State the nominal fill in milligrams and whether it is peptide base or gross powder
Do not reconstitute before shipping unless the laboratory has approved it in writing
Method and reporting scope
Roughly 1.5 to 5 kDa. Identity rests on a whole-spectrum comparison against reference data rather than a single reading, and related substances sit closer to the parent peak.
Area purity by HPLC-DAD, identity against reference spectra, verifiable COA. Reference availability and sample suitability are confirmed before acceptance. Read the method and reporting information for the techniques in your quote.
Price, timing and submission
The starting panel is $299 CAD. Volume rates, optional fees and tax are shown separately. Standard peptide analysis typically takes 5-10 business days after receipt and acceptance; other scopes are confirmed in the quote.
Testing Tirzepatide: what the analysis has to get right
Tirzepatide is one of the most requested samples in Canada, and one of the harder ones to measure well: a long, lipidated peptide where purity, identity and content each need their own evidence.
Tirzepatide is a synthetic 39-residue peptide with a fatty diacid side chain attached through a linker. Its formula is C225H348N48O68 and its average molecular mass is 4813.45 g/mol, more than three times the size of a short peptide such as BPC-157.
That size and the lipid chain change the analysis. The molecule is strongly retained on a reversed-phase column, can adsorb to surfaces and vials, and closely related impurities (truncated sequences, deamidated or oxidised forms) can elute very near the main peak.
What makes Tirzepatide hard to measure
Near-eluting impurities
Related substances differ from tirzepatide by a single residue or modification, so the gradient has to be shallow enough to separate them. A fast method can report a high area purity simply because impurities hide under the main peak.
Identity on a large molecule
Identity is established by comparing the sample against reference spectra, using the techniques that suit the molecule: FTIR-ATR, Raman spectroscopy, capillary electrophoresis with UV detection and UV-Vis spectroscopy. On a peptide this size the comparison is a pattern match across the whole spectrum rather than a single reading, which is why the expected identity has to be named before analysis.
Content needs a reference
Quantitative content is not part of the standard test. It is available on request and depends on calibration against a qualified reference standard on a stated basis. Without one, a milligram figure is an estimate rather than a measurement.
Blends
Products combining tirzepatide with another peptide need each component listed. A combined mass cannot be read as tirzepatide content.
Which test answers which question
Is it tirzepatide?
Identity confirmed against reference spectra by the techniques chosen for the molecule.
How clean is it?
Full-spectrum HPLC with diode-array detection, under a method able to resolve related substances.
Is the label right?
Quantitative content against the labelled amount, on request and subject to reference-standard availability.
Contaminants
Endotoxin or heavy metals are separate tests, requested only if that is the question.
Before you ship
Send tirzepatide as the sealed, lyophilized vial you received. Lipidated peptides can lose measurable material to surfaces once reconstituted, so an opened or dissolved sample describes a different starting point from the label.
Reading a Tirzepatide certificate
Check the identity section names the techniques the confirmation actually rests on, not only a purity figure.
Look for the method conditions (column, gradient, wavelength) beside the purity result, and the chromatogram that comes with every certificate.
If you asked for content, compare the measured amount with the label; purity on its own does not tell you whether the vial holds what it says.
Yes. Canada Peptide Testing accepts mail-in tirzepatide samples from every province and reports identity and HPLC-DAD purity, with a chromatogram, on a verifiable certificate. Quantitative content can be added on request.
Why is tirzepatide harder to test than BPC-157?
It is about three times larger, carries a fatty acid side chain and has closely related impurities, so it needs a separating method able to resolve near-eluting peaks, and its identity comparison spans a whole spectrum rather than a simple pattern.
Does a high purity result mean the dose is correct?
No. Purity is the share of detected signal in the main peak. Whether the vial contains the labelled milligrams is a separate content measurement.
