Peptide testing questions

Peptide analysis is $280 CAD per sample and includes HPLC-UV area purity, identity confirmation against reference spectra, a chromatogram on the certificate, and a verifiable PDF Certificate of Analysis. Volume rates apply automatically: $250 at 3 or more samples, $235 at 6, and $225 at 12. LAL endotoxin is $199 ($189 at 3 or more) and heavy metals $175 per sample. Quantitative content is available on request. Tax, shipping, unusual matrices, method development, and reference materials may be additional.

Peptide analysis combines HPLC-UV chromatographic purity with identity confirmation, which uses the techniques that suit the molecule from FTIR-ATR, Raman, capillary electrophoresis with UV detection, and UV-Vis spectroscopy, compared against reference spectra. The PDF COA states the results, methods, and sample identifiers, and carries the chromatogram for the sample; public reports include QR verification. Quantitative content in milligrams per vial is run on request rather than by default.

Standard identity-and-purity work is generally targeted for 5-10 business days after sample receipt and acceptance. A two-business-day rush option is $199 CAD per sample when capacity and method readiness allow. Method development, complex blends, repeat work, or outside panels can take longer.

The amount depends on the method, compound, concentration, and matrix. Submit the nominal amount and sample form in the request; the laboratory confirms the minimum quantity before mailing. A complete unopened vial is often preferred for content testing.

Yes, after the request is reviewed and accepted. You receive a sample ID, packing slip, approved intake address, and method-specific packing instructions. Unapproved, leaking, hazardous, biological, or controlled materials must not be sent.

Yes, automatically. Peptide analysis is $280 CAD per sample, $250 at 3 or more samples in one request, $235 at 6 or more, and $225 at 12 or more. LAL endotoxin drops from $199 to $189 at 3 or more. The rate is set by the number of samples carrying the same test in one request.

Every released standard panel includes a PDF Certificate of Analysis identifying the sample, methods, dates, results, and limitations. The chromatogram for your sample is on the certificate, and public reports have a verification URL and QR code.

Expected multi-peptide blends such as TB-500 with BPC-157 are accepted for Peptide analysis at the published rate where the standard method is suitable, subject to feasibility review. Tick “Amount unknown” when there is no label claim. An entirely unknown vial is harder: identity here rests on comparison against reference spectra for a compound you name, so tell us what you think it is. Complex blends and confirmatory work may require a custom quote.

HPLC area purity estimates the share of detected chromatographic signal assigned to the main peak. Identity testing compares the material against reference spectra to support that it is the expected compound. Quantitative content estimates how much target peptide is present. None of these measurements should be substituted for another.

Purity is measured chromatographically. Reversed-phase HPLC with ultraviolet detection separates the sample and reports the proportion of detected signal assigned to the principal peak under stated method conditions. Purity is paired with identity confirmation against reference spectra, because a pure sample of the wrong compound is still the wrong compound. HPLC area purity is not the same as peptide content, which is a separate quantitative measurement run on request.

Home reagent kits can indicate that something is present, but they cannot measure chromatographic purity, confirm identity against reference spectra, or quantify how much peptide is in a vial, and they do not produce a traceable record you can show anyone else. Those answers need instrumentation such as HPLC-UV, FTIR-ATR, Raman and capillary electrophoresis run under stated method conditions, with the sample identified and retained by the laboratory.

Third-party testing means the laboratory analysing the sample is independent of whoever supplied or sold it. Canada Peptide Testing does not sell the peptides it tests, so a result is not produced by the seller of the material. Third-party testing does not by itself establish safety, efficacy, or regulatory status: it reports what the stated methods measured in the sample received.

Canada Peptide Testing accepts approved research samples by tracked mail from every Canadian province and territory. Samples are accepted from within Canada only; we do not accept shipments from outside the country. Start online so the compound, matrix, method, required quantity, and mailing instructions can be confirmed before you ship.

A result answers the analytical questions in the accepted scope for the sample received. It does not establish clinical safety, efficacy, sterility unless specifically tested, regulatory status, or consistency across untested units.

A certificate of analysis is the laboratory report for one tested sample. It should identify the laboratory, the sample and lot, the dates, the methods used, and the results, together with a way to verify the report with the laboratory. Different laboratories confirm identity differently, by spectroscopy, by electrophoresis or by mass spectrometry; what matters is that the certificate names the method it used.

Look the report number up on the issuing laboratory’s own website and compare every field with the document. A certificate that names no laboratory, cannot be verified at the source, shows a lot that does not match your vial, or reports purity without identity or content should not be relied on. The free COA checker walks through nine checks.

High-performance liquid chromatography separates the components of a sample so they can be detected one by one. For peptides, reversed-phase HPLC with ultraviolet detection reports area purity: the share of detected signal in the main peak under stated method conditions. Detection is by ultraviolet absorbance at a set wavelength, printed on the chromatogram.

HPLC shows how much of the detected material sits in the main peak, not what that material is. A pure sample of the wrong peptide still looks pure on HPLC alone. Identity is established separately, by comparing the material against reference spectra using FTIR-ATR, Raman, capillary electrophoresis and UV-Vis, chosen to suit the compound. Where a result looks anomalous, confirmatory LC-MS is arranged with a contract laboratory and named on the certificate.

Fill accuracy is the measured content divided by the labelled amount, as a percentage. A 10 mg vial measured at 9.6 mg has a fill accuracy of 96%. It is the number that answers whether the vial holds what the label claims, and it requires quantitative content testing, which is run on request rather than on every sample.

No. Samples are accepted from within Canada only, from any province or territory.

Yes. Reports stay private unless you ask for a shareable verification link or public listing. Contact details are never shown on a certificate or verification page.

Yes. These larger lipidated peptides are accepted for HPLC-UV purity and identity confirmation against reference spectra, subject to reference availability confirmed during scope review. Quantitative content can be added on request.

No. The laboratory does not hold ISO/IEC 17025 accreditation and does not claim it. Methods, conditions and known limits are published for each technique so results can be interpreted on their own terms.

Yes. Choose the name to print on the certificate when you submit the request. A second certificate for the same result under another name is an additional COA at $50 CAD per sample.

Enter the report number on the verification page or scan the QR code printed on the certificate. The page shows the laboratory record and confirms the digital signature matches the document.

Interac e-Transfer carries no fee, and Bitcoin is accepted. Prices are in Canadian dollars, with GST/HST applied for your province. PayPal is not accepted.

Yes. One person submits and pays, and every member receives the same certificate verification link. With ten people, peptide analysis works out to about $28 each before tax.

Kinetic chromogenic LAL endotoxin testing, under the USP <85> framework, measures bacterial endotoxin, a component of certain bacterial cell walls, against a stated reporting limit. Recombinant factor C under USP <86> can be requested instead. It is separate from a microbial count, which measures living organisms and is not offered here, and from sterility testing.

HPLC area purity is the proportion of detected chromatographic signal assigned to the principal peak under the stated method conditions. Content is how many milligrams of the target peptide are in the vial. A sample can have high area purity and still be underfilled. Quantitative content testing is available on request.

Send the sealed, lyophilized vial as received wherever possible. An opened or dissolved sample describes a different starting point from the label, and larger lipidated peptides can lose material to surfaces once reconstituted.

No. Canada Peptide Testing is independent from peptide manufacturers and vendors and does not sell the materials it tests.