Independent analytical testing. Results apply only to the sample received, for research use only.

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Area purity

Seen on certificates, purity reports.

Definition

The area of the principal peak expressed as a percentage of the total integrated detected response in the run, under stated method conditions and at a stated detection wavelength.

Area purity is not peptide content, not milligrams per vial, and not strength. It is normalised against what the detector saw, so material that does not absorb (salt, water, residual solvent) is not in the denominator at all.

Related terms

Peptide content
The measured amount of target peptide in the sample as submitted, determined against a calibrated reference standard and expressed on a stated basis.
Integration
The process of defining where each peak starts and ends on the baseline and calculating the area between.
Detection wavelength
The wavelength at which absorbance is recorded.

About chromatography

Separation, detection, and the figures that come out of a chromatographic run.

Reversed-phase chromatographyGradientRetention timeBaselineResolutionCo-elutionTailing factorTheoretical platesChromophoreIon pairingOrthogonal methodSignal-to-noise ratio

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