Definition
A separation in which the stationary phase is non-polar and the mobile phase is polar, so components are retained according to how hydrophobic they are and are released as the solvent strength increases. It is the default mode for peptides because it separates on a property peptides differ in reliably.
Related terms
About chromatography
Separation, detection, and the figures that come out of a chromatographic run.
Area purityIntegrationBaselineResolutionCo-elutionTailing factorTheoretical platesDetection wavelengthChromophoreIon pairingOrthogonal methodSignal-to-noise ratio