Definition
The use of a mobile-phase additive that pairs with charged sites on the analyte to improve retention and peak shape. Strongly basic peptides tail badly without it, and a tailing peak integrates unreliably.
Related terms
About chromatography
Separation, detection, and the figures that come out of a chromatographic run.
Reversed-phase chromatographyGradientRetention timeArea purityIntegrationBaselineResolutionCo-elutionTheoretical platesDetection wavelengthChromophoreOrthogonal methodSignal-to-noise ratio