Independent analytical testing. Results apply only to the sample received, for research use only.

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Detection wavelength

Seen on chromatogram headers, certificates.

Definition

The wavelength at which absorbance is recorded. Peptides are commonly read near 220 nm, where the peptide bond absorbs regardless of sequence, and near 280 nm where aromatic residues absorb.

A purity figure without its detection wavelength is not interpretable, because the same sample gives different area percentages at different wavelengths.

Related terms

Area purity
The area of the principal peak expressed as a percentage of the total integrated detected response in the run, under stated method conditions and at a stated detection wavelength.
Chromophore
The part of a molecule that absorbs ultraviolet or visible light.
Diode-array detection
An ultraviolet detector that records absorbance across a whole wavelength range at every point in a run rather than at one fixed wavelength.

About chromatography

Separation, detection, and the figures that come out of a chromatographic run.

Reversed-phase chromatographyGradientRetention timeIntegrationBaselineResolutionCo-elutionTailing factorTheoretical platesIon pairingOrthogonal methodSignal-to-noise ratio

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